best enzyme cleaner for cat urine enzyme cleaner with text

7 Best Enzyme Cleaners for Cat Urine

As cat parents, you must’ve dealt with your pet’s bathroom accidents at one time or another. Although we absolutely love our furry little friends, the nasty pee smell that lingers on the carpet or the sofa where they had peed could be the worst thing ever. 

If you’ve had no success in getting rid of the smell and stains of cat pee with baking soda, hydrogen, vinegar, or soda water, then what you really need to do is find the best enzyme cleaner for cat urine.

Enzyme cleaners break down organic matter and effectively eliminate stains and odors without exposing your family or pet to harmful chemicals. Here’s everything you need to know about them.

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What Makes Enzyme Cleaners for Cats So Effective?

enzyme cleaner for cat urine urea on hand with gloves

A cat’s urine contains several components, including sodium, pheromones, creatinine, urea, bacteria, electrolytes, urobilin, etc. Although most of these components are easy to eliminate with common household cleaning products, it’s the uric acid and salts in your cat’s pee that bind tightly to whatever surface it touches. 

When your cat pees, the uric acid sticks to fibers, and as time passes, it becomes harder to remove. Although you might not notice the smell of the urine, your cat has sharper senses.

That’s why it’ll go back to the same spot repeatedly, resulting in spraying and repeat incidents all over your home.  

Common household cleaners aren’t capable of removing uric acid. Every time uric acid comes into contact with water, it changes into crystals which are quite hard to remove. Although uric acid can break naturally over time, it can take many years.

This is where enzymatic cleaners come in. They can effectively remove all pee stains by transforming the uric acid into carbon dioxide and ammonia, which evaporate, leaving behind no residual crystals.

7 Best Enzyme Cleaners for Cat Urine

The large number of cleaners on the market can make it quite difficult to choose an enzyme cleaner that’s effective in removing the stain and smell of cat urine. To help you out, here’s a list of the best cat urine removers that promise the best results.

enzyme treatment for cat urine hepper banner
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After careful evaluation, we find the Hepper Advanced Bio-Enzyme to be the best overall cat urine cleaner. Its powerful and fast-acting formula can penetrate even the most stubborn stains and smells at the deepest molecular levels and completely eliminate them from the source.  

This neutrally-scented versatile spray works with not only urine spatters but also poop, drool, and vomit as well. You can use it on almost any surface, including carpets, floors, car seats, furniture, bedding, and wood. It also comes in quite handy in freshening up areas with foul smells.    

Hepper Advanced Bio-Enzyme cleaner is very convenient to use. You don’t need to mix it with water, neither does it require any scrubbing. Simply apply the cleaner on the stain, and before you know it, the stain and smell will be completely eradicated. It’s also safe to use around pets and kids since it doesn’t contain any unpleasant or dangerous chemicals to get the job done.


  • Multipurpose
  • Safe for the environment
  • Neutral smell
  • Great for carpet cleaning
  • Removes odors from fabrics without causing any damage


  • May not stop your pet from going to the same spot
enzyme detergent for cat urine sunny and honey bottle image with mint leap

If you’re looking for an enzyme detergent for cat urine that makes cleanup fast and easy, then the Sunny & Honey Enzyme Cleaner is for you.

It is quite popular among cat owners as it takes care of pet messes very easily. You can use it for quick spot cleaning in case of small incidents, as it works wonders for feces, vomit, and other organic liquids. 

This multipurpose cleaner is suitable for multiple surfaces, including leather, tile, concrete, rugs, car upholstery, pet beds, and hardwood. In addition, it works on wooden decks and artificial grass, and doesn’t just mask the smell but completely removes the cat pee smells from around your home.

Made from high-quality ingredients, this cleaner is biodegradable, non-toxic, and scented with essential oils. Plus, it’s Leaping Bunny certified, so you can use this product without any worries.


  • Available in three scents
  • Works on all surfaces
  • Safe for use around pets and kids
  • Well-known and established brand 
  • Biodegradable and non-toxic


  • A few users have found the smell to be overwhelming
Simple Solution Cat Extreme Pet Stain and Odor Eliminator Bottle Image with Gradient Background
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The Simple Solution Extreme Remover is an economical yet powerful enzyme cleaner that works efficiently on various water-safe surfaces.

It is made in the USA, and uses the 3X pro-bacteria and enzyme formula to eliminate stains and neutralize odors in no time. The spray nozzle has three different settings – mist, foam, and stream, allowing it to target different problems more effectively.  

This alcohol-based formula enzyme cleaner is the best for carpet cleaning, and can also be used on bedding, upholstery, toys, and other fabrics without any worries.

In addition to urine stains, it helps take care of vomit and feces and leaves the surfaces clean. You can use it around your pets and kids as it’s safe to use. 

Overall, it’s perfect for pet parents with varying preferences since it targets the toughest odors and stains by penetrating deep into the fibers and breaking down the stains. It also helps discourage repeat spraying.

Last update on 2024-07-19 / Affiliate links / Images from Amazon Product Advertising API


  • Affordable
  • Professional strength formula
  • Three-in-one nozzle function
  • Made in the USA
  • Eliminates stains and odor from various surfaces
  • Works on cat feces and vomit as well
  • Non-toxic and safe to use around children and pets


  • Some people may find the smell a little strong

Best for Carpet Cleaning: Rocco & Roxie Stain and Odor Remover

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If your living room carpet is a mess or your bedroom rug is always a target, then you definitely need to try out the Rocco & Roxie cleaner. This is one of the best enzyme cleaners for carpet cleaning, and comes with the approval seal of the Carpet and Rug Institute

The natural enzymes in this advanced stain remover are activated when they come in contact with stains and odors. It feeds on organic matter and ammonia until they are completely removed.

This formula is color-safe and chlorine-free, which makes it safe to use around pets and kids. It doesn’t contain any dangerous propellants and doesn’t leave behind any residue. 

This cleaner eliminates all stains and odor from stinky yellow pet urine and vomits to feces while protecting your carpet from any damage.

It also works great on all surfaces where stains happen, including litter boxes, floors, clothing, carriers, kennels, furniture, and all pet living and sleeping areas. 

The best thing about this pet stain remover is that it comes with a 100% satisfaction guarantee. You’ll receive a full refund if it doesn’t completely eliminate the stains and odors.


  • Fresh, minty smell
  • Chlorine-free and color-safe
  • Suitable for all types of carpets or rugs
  • Works on both old and new stains 
  • Safe and effective
  • Professional-strength bio-enzymatic formula
  • Carpet and Rug Institute approved


  • Application process is a bit lengthy
BUBBAS Super Strength Enzyme Cleaner Bottle Image with Brown Background Color

If a standard spray bottle isn’t enough to clean all the pet stains around your home, then you need to consider BUBBA’S Super Strength Commercial Cleaner. This commercial-grade solution can eliminate the worst urine smells and target the most stubborn stains.

Despite being one of the most powerful formulas on our list, this cleaner is safe for use on all fabrics. It doesn’t contain any chemicals that’ll cause the colors of your upholstery to fade or stain. 

This multipurpose product is also excellent for spot treatment. Just spray the cleaner over the stain and wait a few minutes. The enzymes will activate once they come into contact with organic matter and ammonia crystals and work until they remove all traces of feces, vomit, and urine.

You can also spray this cleaner on clothes and toys before throwing them into the washing machine.     

This super-strength cleaner from BUBBA can also be used to tackle floors efficiently. Mix it with water and clean your tiles and hardwood floors easily. For old stains, you might have to use the cleaner repeatedly until all the stains are eliminated.


  • Suitable for use on multiple surfaces, including carpets, floors, furniture, etc.
  • Safe to use around pets and kids
  • No chemicals added
  • Excellent for spot treatment
  • Approved by the Carpet and Rug Institute
  • Comes with money-back guarantee


  • May leave stains on light-colored fabrics
Angry Orange Stain Remover Bottle with Orange Gradient Shade Background
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The next enzyme cleaner on our list is one that can handle all types of messes, whether they are because of your dog or cat. It can effectively tackle all stains, including urine, vomit, and feces, and works on most surfaces, from hardwood floors to turf, fabric, carpet, and more. 

Since this cleaner is made from citrus enzymes, it gives your home a fresh feel. It just doesn’t mask the smell but destroys it upon contact, providing you with up to 80 hours of freshness.

Plus, it doesn’t contain any parabens, phthalates, sulfates, bleach, or peroxide, making it safe for use around pets and kids.


  • Made in USA
  • Can be used on multiple surfaces
  • Fresh, citrusy scent
  • Made from natural ingredients


  • Users aren’t happy with the packaging as the spray leaks
Nature’s Miracle Hard Floor Cleaner Bottle Image with Green Background
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The last product on our list is from Nature’s Miracle. Pet owners trust this cleaner to eliminate stains and get rid of odors effectively. It’s not just the ideal product for eliminating the smell of urine and the stains left behind by urine and fecal matter but also works on grease, fats, oil, and other organic substrate stains. 

You can also use this cleaner for removing deep-set and new stains and odors from vinyl, linoleum, sealed wood, ceramic tile, and concrete. Its bio-enzymatic formula cleans deeply without damaging the natural floor finish.

It’s safe to use around pets and kids, but be sure to follow the storing and handling instructions provided on the bottle.

Last update on 2024-07-20 / Affiliate links / Images from Amazon Product Advertising API


  • Eliminates deep-set stains from all types of floors
  • Safe and convenient to use
  • Doesn’t require rinsing 
  • Dual-action formula effectively removes stains and odors
  • Comes with money-back guarantee


  • Some users find it ineffective against pet odor

Best Enzymatic Cleaner for Pet Odor: Buying Guide

Best Enzymatic Cleaner for Pet Odor- Buying Guide - Image With Guide Text

Although looking for the best enzyme cleaner can be a tedious task, it’s quite worth the time and effort. It’ll effectively remove cat urine smells and stains and keep your home tidy and fresh.

Here are some essential factors that you should keep in mind when searching for an enzymatic cleaner:


Enzyme cleaners come in various smells, and you should choose one according to your preference. Remember that cats aren’t overly fond of intense smells, so it’s best to keep away from scents like lavender and citrus.

Easy to Apply

Easy to Apply Cartoon Character Man Stand with Easy Text Banner

Choose a stain remover that’s convenient to apply. Enzyme cleaners that come in spray bottles are more popular as they ensure precise application, and are perfect for small areas and hard-to-reach places. 

But if you need to clean a larger area, it’s best to use powder cleaners or liquid cleaners that come in large containers. You’ll need to mix these cleaners with water before you can treat the stains.

However, this is not a problem as most of these products come with detailed instructions on the amount of water you need to add.

Safe Ingredients

Safe Ingredients Safe Text on a Paper in a Hand

Another thing to keep in mind when choosing an odor remover is that it should be safe to use around children and pets. It’s best to choose an unscented enzymatic cleaner if your cat or a family member is prone to allergies. 


Is your cleaner able to remove old stains, or does it work only on new stains? Although most companies claim that their product can remove tough stains, it’s best to read the packaging carefully before purchasing so that you can buy a cleaner that fits your needs. Reading reviews by other users can also help you make a better decision.

Versatile Use

You should also make sure that the odor eliminator is versatile and can be used on multiple surfaces. If your enzymatic cleaner isn’t fabric-friendly, then you’ll be able to use it only on hardwood floors, and you’ll have to invest more to get soemthing for your carpets, bedding, etc.   

Frequently Asked Questions: Best Pet Stain and Odor Eliminator

Which enzyme works best on cat urine?

The enzyme protease breaks down the proteins in urine effectively, so make sure that it’s present in the enzyme cleaner you choose.  A protease enzyme cleaner works against other bodily fluids like vomit and feces as well.

Can enzyme cleaners get rid of old and tough cat urine stains?

Yes, enzymatic cleaners break down the urine stains, even if they are old. Make sure that the surface is damp before applying a generous amount.

Leave it for at least an hour. The cleaner will break down the uric acid, completely eliminating the odors and stains.

What’s the primary benefit of using enzyme cleaners?

The primary benefit of using enzyme cleaners is that they eliminate the toughest stains and odors without much difficulty.

Common household cleaners may remove urine odor, but it’s usually a temporary fix. Your cat will pee in the same place again and again unless you use an enzyme cleaner. 

Are there any other uses of an enzymatic cleaner?

You can use an enzymatic cleaner to clean almost everything. In addition to eliminating cat urine, odor, feces, and vomit, you can use them to clean different stains like stubborn chocolate, oil, wine stains, and much more.

How long will it take for an enzyme cleaner to remove a stubborn stain?

The amount of time it takes for an enzyme cleaner to remove cat urine stains varies from product to product. Some are strong and need only 15 minutes to work, while others may take up to an hour. For stubborn pet stains, it’s best to leave the solution for a longer time.

Final Thoughts

If you’re having a hard time getting rid of your pet’s odor or stain, then enzyme treatment for cat urine can make your job easier.

Enzyme cleaners are safe to use around kids and pets, affordable, protect you from bad microbes, and better for the environment. The products reviewed above offer something for every pet parent, so be sure to choose one that suits your requirements perfectly.


Picture of Evelyn Baxter, Writer and Cat Expert

Evelyn Baxter, Writer and Cat Expert

Evelyn is an animal advocate, cat expert, and the proud mom of 2 rescue cats, Sugar, a beautiful fluffy tabby and Beebee, a black, fluffy beauty. Evelyn has studied animal care while volunteering at her local shelter. She loves all animals and wants to share the animal knowledge she has been lucky enough to gain together with knowledge from other experts with cat parents across the world.